4/3/2024 0 Comments Skyrim sexlab aroused reduxPlease take your screen archery to /r/SkyrimPorn. Screenshots do not contribute to the discussion of modding or mod creation.No Screenshots (except to ask for help).Memes and gameplay stories do not contribute to this whatsoever. This is a sub centered around the discussion of modding and creating mods.Sexual content ( galleries / screenshots that involve sexual acts) is not. Posting about and discussing the use of these is fine. There are a wide variety of body meshes and armors that are NSFW.When you sign up for Nexus you agree to a ToS that says you will not do these things. No we don't care about your views on the finer points of copyright law.This includes sharing/rehosting a mod without the original author's express permission.If you promote piracy or seek help in modding a pirated game you will be banned. If you submit a link to or explain how to obtain pirated material you will be banned.We have worked hard to cultivate a positive environment here and it takes a community effort.Hop in and chat with other members of the community! Modding Skyrim: Modder's Edition Use this and the CK Wiki together to start creating!.Everything you need to know about the Creation Kit!.Youtube channels related to skyrim modding.Community Discussions based around various themes.Tips for figuring out what's causing your issues! Maintained by /u/Thallassa.A community curated list of 'essential mods.'.A comprehensive list of many guides and resources covering a wide variety of topics. If you want to know the main advantages and disadvantages of SSE so you can decide what to play, here they are.If you are brand new to modding work through this before asking where to start.You may not post about questions that are answered in the FAQ.Submit Help Post Submit Text Post Posting Rules FiltersĪdd, ,, , or in the title of your post so posts can be filtered! Unfilter Using Mods
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Do that and after you are logged in, you will see your Samsung Notes will start appearing in the feed.Īs mentioned earlier, the feed shows Sticky Notes, OneNote pages, and Samsung Notes. You might be asked to sign in again to your Microsoft account. Inside the notebook, click on Feed at the top-right corner.Ĥ. You will be greeted by your list of notebooks. Make sure to use the same account that you used on your Samsung Notes app.Ģ. Open /hrd in a browser on your MacBook and log in to your Microsoft account. You can do it from OneNote web or Outlook web. Now, there are two ways to access your note in the OneNote feed. Tip: Know how to access Samsung Reminders on a computer. You can also change the folders to sync, sync internet type, and enable the sync while roaming feature. For that, go to Samsung Notes Settings and tap on Sync to Microsoft OneNote text. But if that doesn’t happen, you can manually press the Sync Now button or change sync settings. Typically, your notes should start syncing to the OneNote feed. You can also select any other custom folder. Tap on Folders since it has all the notes generally. Once logged in, you will be asked to select the folders that you want to sync to OneNote. Tap on the Get Started button and log in to your Microsoft account.ĥ. Tap on the three-bar icon at the top and press the Settings icon.Ĥ. Open the Samsung Notes app on your phone.Ģ. Connect Samsung Notes to OneNoteįollow these steps to link Samsung Notes to your Microsoft account:ġ. To do so, first, you need to connect your Samsung Notes to OneNote using your Microsoft account and then view the notes in the OneNote feed. But don’t worry, you can still access your Samsung Notes using OneNote web or Outlook web versions. 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Once you have an Google Cloud bucket ready to go, navigate to your Couchdrop Portal and navigate to admin then select storage connectionsin the top navigation menu.Ĭlick the + icon to add a new connector and select Google Cloud from the dropdown Finish creating the account by selecting Done.Provide the user the Storage Admin role.The software supports FTP, FTPS, and SFTP transfers. Select the project your bucket resides under SolarWinds Serv-U Managed File Transfer Server (FREE TRIAL) SolarWinds Serv-U Managed File Transfer Server is one of the most versatile FTP Servers on the market. Navigate to IAM & Admin -> Service Accounts.a free file-transfer manager that supports Amazon S3 and Google Cloud but, oddly enough, not FTP, SFTP or FTPS. There are three steps to configure Google Cloud Storage for CouchdropĬonfiguring Google Cloud for SFTP Create a storage bucket Most decent FTP clients can connect to servers using FTP, FTPS and SFTP. Linking Google Cloud with Couchdrop requires an existing Google Cloud account, a bucket and your JSON service account file. Gemma O'Brienįocusing on typography, illustration and graphic design, Sydney-based Gemma O'Brien's typographic works have attracted the attention of some of the world's best known brands, including Adobe and Diet Coke. They've also written for Shillington's blog and have an array of inspiring projects to peruse. Smack Bang Designs is an acclaimed design studio in Sydney that provides empowering brand identities for clients across Australia and the rest of the world. Josh Nychuk is a Canadian graphic designer based in New York and part-time lecturer for Shillington on Madison Avenue. She's also a part-time lecturer at Shillington in London. London illustrator and designer Anna Dunn has a cheerful, bold and colourful style that is instantly recognisable and is guaranteed to put a smile on your face. With a strong focus on typography and simplicity, her work will appeal to the minimalist in you. Part-time lecturer for Shillington in London, Emma Stokes lovingly art directs and crafts brand identities and web designs for a range of clients across the UK. George SimkinĪnother part-time lecturer of Shillington's, George Simkin is a London-based freelance designer and illustrator who brings a bold and playful approach to typography to both branding and editorial work. She's also a lecturer at Shillington in Brisbane. She especially enjoys branding and typography, and gets to work largely with not-for-profit organisations. Shanti is a graphic designer who works commercially with a beautiful studio in Sydney called Bug Communication. His award-winning portfolio, which has garnered the attention of numerous brands and award panels, is testament to his hugely imaginative style, inspired by daydreams, travel, and an obsession with colour. Spencer Harrison, otherwise known as Spenceroni, is a Melbourne-based graphic artist, designer, and Shillington lecturer that specialises in vibrant, energetic pattern design and illustration. Based in New York, he runs his own studio and is also a part-time lecturer at Shillington on Madison Avenue. The optimum of cool, Saxon Campbell has built quite a reputation for his branding work for fashion and retail brands with clients including P&Co, Frockhub and The Monster Cycle. Add this to your bookmarks for next time you need some inspiration! 1. Check out our essential list of graphic designers on Behance, starting with some of our very own lecturers from around the world. From masterful typographers and top digital artists to the finest crafters of logos and packaging designs, at Shillington we are constantly on the lookout for the latest talent. 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This game was tested and reviewed on Xbox One. To also now be able to play this multiplayer as well can only make the game more interesting. Although it has taken a while for it to finally make its way to the Xbox One, fans of the first game or those into isometric RPG’s will be happy to tuck into this game as there is plenty of content and variety to keep things interesting. It keeps true to what made the first Torchlight a decent RPG and built on it. With 3 others and take on the game together with friends or withĪll in all, Torchlight 2 doesn’t explore new territory, but it doesn’t need to. Playing by yourself there is also a multiplayer option to jump in To equip better gear and explore different skills. Through the quests and the defeat your enemies you will level up yourĬharacter to assign more points to your skills and stats to allow you Your companion being away for a small period. What more is when you have accrued too much rubbish youĬan send your pet back to town to sell all the junk, at the cost of Some good, some rubbish, but plenty of it to keep you The maps and quests as you give the enemies a good seeing to, theyĭrop loot. Pleasantly keep you busy from the main quest for a while. You occasionally will find sub-quest givers which will Large, and they even contain some sub dungeons so there is plenty toĮxplore. Then, when you venture out to the maps, you will find they are quite Which have the shops to buy items with, storage to store yourįavourite loot or higher level loot for later and your quest givers. Launch an attack at your pet by accident – it’s hard to distinguishīetween friend and foe when the carnage hits. When enemies start to fill the screen it becomes a bit too easy to Your not tackling enemies alone, even in single-player mode. With your pet fighting alongside you, it’s nice to know To the buttons of your choice, allowing you to tailor this yourselfįor comfort. The standard for this genre, with skills, potions and items assigned Game itself looks great and plays very nicely. Style has not grown stale – there is still a demand for it, and this Not over-complicated and can be considered a hack n slash, but this Game plays in the same fun style as the first game did. Having played for a fair few hours now, I have only died onceĪnd that was down to carelessness rather than great difficulty. Normal – the default setting – and found the challenge not too Hardcore option for those who need that extra challenge. There are many other pets to choose from as well, so the variety isįew difficult levels, from casual to elite, as well as an additional Like pet lsuch as a headcrab, chakawary, molten imp and a yapper. You can have a badger, owl, hawk, panda andĬouple of breeds of dog to name but a few. I found there to be more variation on the pet than there isįor your own class. Provides you with a pet companion to take along with you on your But I was not disappointed, as the skills made upįor it – for example, a robot droid who sends out a healing pulse. Steampunk weaponry or armour and instead, I got a massive wrench toīeat enemies with. I chose the engineer thinking they would have all kinds of Variety makes you want to sample each class to see what works bestįor you. With 4 interesting choices of character an Engineer, an Outlander, aīerserker and an Embermage. Torchlight has now been corrupted by the ember, and it’s up to you to The story is just as complex and confusing. The hack-n-slash, lootingĪnd the wonderful visuals are just as great as before. Manages to carry on the magic of the first. The sequel to a decent isometric dungeon-crawling RPG, it II finally makes its way to the Xbox One after being out on PC sinceĢ012. – Yunho said Seonghwa is his favorite fashion icon/model. They both auditioned for the company without the other knowing and met at the audition. – Yunho and Mingi have known each other before joining KQ Entertainment. – His role models are TVXQ‘s Yunho and EXO‘s Kai. – His specialties are his dance, his vocal and gaming. – His hobbies are playing with members, gaming and sport. – Nicknames: Yunhogizer, Golden Retriever, Big bear. – Yunho was the second member to join KQ Entertainment. Yunho Stage Name: Yunho (윤호) Birth Name: Jeong Yun Ho (정윤호) Position: Main Dancer, Vocalist Birthday: March 23rd, 1999 Zodiac Sign: Aries Height: 186 cm (6’1”) Weight: – Blood Type: A MBTI Type: ENFJ-A Representative Emoji: □ – After Kingdom: Legendary War, he said that he became close to THE BOYZ‘s Juyeon and Stray Kids‘ Lee Know. He is also friend with P1Harmony‘s Soul. – Seonghwa’s fans are called “ddeongwife” (떵와이프). – He comforts the members when they need it. – Seonghwa is called the best loooking, dancingamd best singing by member because of his versatility. He played the role of Seyoung, a SPARKLING‘s member. – In 2021, Seonghwa, along with Yunho, San and Jongho, debuted as an actor in the Korean drama ‘ Imitation‘. – He is fluent in English and can also speak basic Japanese. – Seonghwa auditioned for MIXNINE but didn’t pass. – Seonghwa’s microphone color is silver because he wanted to get one since he was a trainee and he thinks it’s cool. – He falls asleep right away when he drunk. – He thinks Yunho is the funniest member. – His role models are Shinee‘s Taemin and EXO‘s Kai. – His favorite colors are Black, Silver and Blue. – Seonghwa likes to play Animal Crossing. – He smells the best in the group according to the members. – In High School, he was the head of the dance club. – His hobbies are watching dramas, building action figures, cleaning, ASMR and playing games. However, he recently said that each members has grown and that he feel like he and Hongjoong doesn’t have to take on these roles anymore. – Seonghwa is considered the mom of the group. – Fans said he look like Toothless from the movie ‘How to Train Your Dragon’. – Seonghwa was the fifth member to join KQ Entertainment. Seonghwa Facts: – He was born in Jinju, Gyeongnam, South Korea. Seonghwa Stage Name: Seonghwa (성화) Birth Name: Park Seong Hwa (박성화) Position: Vocalist, Rapper, Performer, Visual Birthday: April 3rd, 1998 Zodiac Sign: Aries Height: 178 cm (5’10″) Weight: 61 kg (134 lbs) Blood Type: O MBTI Type: ENFJ-T (His previous result was ESFJ) Representative Emoji: □ / ⭐️ – One of Hongjoong motto is “Don’t waste your calories with food that aren’t delicious.” Show more Hongjoong facts… – Hongjoong is close friends with the soloist MADDOX, EDEN, Stray Kids’ Bang Chan, soloist and former UNIQ and X1 member WOODZ, actor Yoo Insoo, TREASURE’s Choi Hyunsuk and CIX’s BX. – Hongjoong’s fans are called “hongsami” (홍삼이). The money earned from the event was then donated to charity. – Hongjoong held his first film photo exhibition named ‘From November 7, 1998’ at GALLERY99 in Mapo-gu, Seoul from November 1 to November 7, 2022. – Hongjoong and Yunho are the hosts of MBC’s Idol Radio Season 3. He ranked 42nd overall and was eliminated in episode 10. – Hongjoong’s microphone color is white because he thinks it goes well with all the colors. – He is fluent in English and can also speak basic Japanese and Spanish. – As of March 2023, Hongjoong has 92 KOMCA credits. – He said he likes may UK artists like David Bowie, One Direction and Harry Styles. – Seonghwa and Hongjoong hate mint-chocolate. – His favorite colors are Red and Yellow. – He has been actively supporting campaigns such as Polished Man, Dear Heart and Happy Hippie Ring. – Hongjoong has two tattoos: one on his ankle featuring the word ‘faith’ with wings and another on his inner right arm saying ‘NO 1 LIKE ME’. – His hobbies are listening to music and composing. – Hongjoong is considered the dad of the group. – Nicknames: Hong-leader, Mini-Hong, Korea Big Minion, Pokjoong. – If the ATEEZ’s members call Hongjoong « Leader » instead of « Captain », they have to give him 50,000 wons. – The CEO gave him the name of ‘Captain’ because of their worldview/concept. – Hongjoong was the first to join KQ Entertainment. – He has an older brother named Bumjoong who is a contemporary dancer and an actor. – He was born in Anyang, Gyeonggi-do, South Korea. Hongjoong Stage Name: Hongjoong (홍중) Birth Name: Kim Hong Joong (김홍중) Position: Captain, Main Rapper, Vocalist, Composer, Center Birthday: November 7th, 1998 Zodiac Sign: Scorpio Height: 172 cm (5’8″) Weight: – Blood Type: B MBTI Type: INFP-A Representative Emoji: □ Twitter: ateezofficial / ATEEZstaff / ATEEZofficialjp (Japan) Instagram: ateez_official_ / from_the_witness ATEEZ Fandom Name: ATINY (a combination of ATEEZ + DESTINY) ATEEZ Fandom Color: –ġ: Seonghwa, San, Mingi 2: Hongjoong, Wooyoung, Jongho 3: Yunho, Yeosang 4/3/2024 0 Comments What chromosome is SCIDS onConstruction of targeted recombineering plasmids The position of the lac promoter:: gfp insertion site is given in base pairs with respect to the coordinate system origin ( 32). MATERIALS AND METHODS Strains and plasmidsīacterial strains and plasmids used in this study are listed in Supplementary Table S1. We identified several factors that impact upon gene expression, including processes that are involved with chromosome structuring and organization. We observed substantial position-dependent variation of promoter activity that is mediated at the point of transcription and is unrelated to gene dosage. coli by inserting a transcription reporter cassette at different targeted positions in the genome. Here we have re-addressed chromosomal position effects in E. Only a handful of similar studies have been conducted in bacterial systems, with all attributing minor differences in gene expression to gene dosage effects: the correlation between the increase in gene expression and the proximity of the gene to the origin of replication ( 23–31). Furthermore, expression levels were reflective of the local chromosomal environment: being attenuated in lamina binding domains and areas of compaction, and enhanced when located proximal to active genes. ( 22) analyzed ∼27 000 reporter gene integrations in mouse embryonic stem cells and demonstrated that expression varied across the genome by more than 1000-fold. In eukaryotic systems, it is well established that the expression of individual genes can be greatly affected by chromosomal position ( 18–21). These foci are located in discrete areas of the cell, indicating that some transcription events occur at particular locations and that the specific chromosomal position and spatial organization of genes may be important for maintaining control of gene expression ( 13, 16). Transcriptionally active regions are thought to be extruded to the periphery of the nucleoid where they are engaged by transcription foci, dense in RNA polymerase (RNAP) ( 11–17). Termed transcriptionally silent Extended Protein Occupancy Domains (tsEPODs), these domains overlap with regions bound by NAPs, but neither the precise protein organization nor whether tsEPODs contain predominantly poor promoters, or active promoters silenced by the associated proteins, is known.ĭespite being highly compacted, the nucleoid remains accessible for cellular processes such as transcription and replication. Highly proteinaceous transcriptionally silent regions of the chromosome have been identified as potential organizational hubs that may insulate the topologically isolated loops and macrodomains ( 10). All NAPs influence DNA conformation: with some binding predominantly within one macrodomain and others binding ubiquitously throughout the genome ( 6–9). Organization of the nucleoid is mediated by DNA supercoiling, macromolecular crowding and by a number of nucleoid associated proteins (NAPs), although the precise impact of each on the overall structure is not fully understood. Comprised of chromosomal DNA, protein and RNA, the nucleoid in Escherichia coli and Salmonella is arranged into topologically isolated loops, each ∼10 kb in length, which are further organized into four spatially isolated, structured macrodomains and two non-structured regions ( 3–5). The nucleoid is a highly compact and organized structure occupying the majority of the intracellular cytoplasmic space in most bacteria ( 1, 2). Taken together, our findings identify a tier of gene regulation above local promoter control and highlight the importance of chromosome position effects on gene expression profiles in bacteria. Rather, the differences in gene expression occur predominantly at the level of transcription and are mediated by several different features that are involved in chromosome organization. These effects were not due to differences in gene copy number, caused by partially replicated genomes. At some positions, expression was more than 3-fold higher than at the natural lac promoter locus, whereas at several other locations, the reporter cassette was completely silenced: effectively overriding local lac promoter control. coli lac promoter driving expression of gfp, varies by ∼300-fold depending on its precise position on the chromosome. Here, using Escherichia coli K-12, we demonstrate that expression of a reporter gene cassette, comprised of the model E. In eukaryotes, the location of a gene on the chromosome is known to affect its expression, but such position effects are poorly understood in bacteria. |
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